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zikv vlps  (Native Antigen Inc)


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    Native Antigen Inc zikv vlps
    Zikv Vlps, supplied by Native Antigen Inc, used in various techniques. Bioz Stars score: 92/100, based on 8 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/zika+virus+vlp/us12534517-955-0-2?v=Native+Antigen+Inc
    Average 92 stars, based on 8 article reviews
    zikv vlps - by Bioz Stars, 2026-07
    92/100 stars

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    Reactivity of <t>anti-ZIKV</t> MAbs. (A) Reactivity of anti-ZIKV MAbs with ZIKV and DENV virus-like particles <t>(VLP)</t> using Luminex assay. ZIKV- and DENV-VLP were conjugated to MagPlex beads (Luminex) and 10,000 beads/mL of these beads mixed with 10 μg/mL to 0.0002 ng/mL anti-ZIKV MAb 102-1, 242-3, 270-12, 289-3, 306-2, 11-3, 278-11, 78-2, and 4G2 for 90 min at room temperature. (B) Western blot analysis of anti-ZIKV MAbs. ZIKV-VLP were heat denatured under nonreduced conditions at 70°C for 5 min. Samples (left, 27 ng; right, 240 ng) were electrophoresed by Wes capillary and detected by 10 μg/mL anti-ZIKV MAb clones. Left, MAb 102-1, 242-3, 270-12, 289-3, 306-2, 78-2 and 11-3. Right, 278-11. M, molecular weight marker. The estimated molecular mass of ZIKV E protein, 55 kDa; prM protein, 23 kDa.
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    Reactivity of anti-ZIKV MAbs. (A) Reactivity of anti-ZIKV MAbs with ZIKV and DENV virus-like particles (VLP) using Luminex assay. ZIKV- and DENV-VLP were conjugated to MagPlex beads (Luminex) and 10,000 beads/mL of these beads mixed with 10 μg/mL to 0.0002 ng/mL anti-ZIKV MAb 102-1, 242-3, 270-12, 289-3, 306-2, 11-3, 278-11, 78-2, and 4G2 for 90 min at room temperature. (B) Western blot analysis of anti-ZIKV MAbs. ZIKV-VLP were heat denatured under nonreduced conditions at 70°C for 5 min. Samples (left, 27 ng; right, 240 ng) were electrophoresed by Wes capillary and detected by 10 μg/mL anti-ZIKV MAb clones. Left, MAb 102-1, 242-3, 270-12, 289-3, 306-2, 78-2 and 11-3. Right, 278-11. M, molecular weight marker. The estimated molecular mass of ZIKV E protein, 55 kDa; prM protein, 23 kDa.

    Journal: Journal of Virology

    Article Title: Somatic Hypermutation and Framework Mutations of Variable Region Contribute to Anti-Zika Virus-Specific Monoclonal Antibody Binding and Function

    doi: 10.1128/jvi.00071-22

    Figure Lengend Snippet: Reactivity of anti-ZIKV MAbs. (A) Reactivity of anti-ZIKV MAbs with ZIKV and DENV virus-like particles (VLP) using Luminex assay. ZIKV- and DENV-VLP were conjugated to MagPlex beads (Luminex) and 10,000 beads/mL of these beads mixed with 10 μg/mL to 0.0002 ng/mL anti-ZIKV MAb 102-1, 242-3, 270-12, 289-3, 306-2, 11-3, 278-11, 78-2, and 4G2 for 90 min at room temperature. (B) Western blot analysis of anti-ZIKV MAbs. ZIKV-VLP were heat denatured under nonreduced conditions at 70°C for 5 min. Samples (left, 27 ng; right, 240 ng) were electrophoresed by Wes capillary and detected by 10 μg/mL anti-ZIKV MAb clones. Left, MAb 102-1, 242-3, 270-12, 289-3, 306-2, 78-2 and 11-3. Right, 278-11. M, molecular weight marker. The estimated molecular mass of ZIKV E protein, 55 kDa; prM protein, 23 kDa.

    Article Snippet: DENV-1-VLP (Nauru/Western Pacific/1974), DENV-2-VLP (Thailand/16681/84), DENV-3-VLP (Sri Lanka D3/H/IMTSSA-SRI/2000/1266), DENV-4-VLP (Dominica/814669/1981), ZIKV-VLP (Suriname Z1106033), and ZIKV E protein (Suriname Z1106033) were purchased from The Native Antigen Company (Oxford, UK).

    Techniques: Luminex, Western Blot, Clone Assay, Molecular Weight, Marker

    Summary of characterization of anti-ZIKV MAbs

    Journal: Journal of Virology

    Article Title: Somatic Hypermutation and Framework Mutations of Variable Region Contribute to Anti-Zika Virus-Specific Monoclonal Antibody Binding and Function

    doi: 10.1128/jvi.00071-22

    Figure Lengend Snippet: Summary of characterization of anti-ZIKV MAbs

    Article Snippet: DENV-1-VLP (Nauru/Western Pacific/1974), DENV-2-VLP (Thailand/16681/84), DENV-3-VLP (Sri Lanka D3/H/IMTSSA-SRI/2000/1266), DENV-4-VLP (Dominica/814669/1981), ZIKV-VLP (Suriname Z1106033), and ZIKV E protein (Suriname Z1106033) were purchased from The Native Antigen Company (Oxford, UK).

    Techniques: Luminex, Western Blot

    Kinetic analysis of anti-ZIKV MAbs. Kinetic analysis was conducted by Octet HTX (Sartorius). Anti-ZIKV MAbs were conjugated to an amine-reactive 2nd generation (AR2G) biosensor at 0.1 to 0.3 μg/mL, and the association constant ( k a ) was measured over 0 to 900 s and dissociation constant ( k dis ) for 1,200 s for ZIKV-VLP or E proteins at various concentrations. (A) MAb 289-3 and 102-1 to ZIKV-VLP, 3.3 to 16.7 nM. (B) MAb 289-3 and 102-1 to ZIKV E proteins, 6.67 to 33.3 nM, red line, fitting pattern. (C) k a / k dis plot for anti-ZIKV MAbs to ZIKV-VLP and ZIKV E proteins. Blue, MAb 78-2, 289-3, 306-2 and 278-11; red, MAb 102-1, 242-3. 270-12, and 11-3. k a and k dis values were calculated by two runs, and the average values are shown. Values (nM) for equilibrium dissociation constants ( K D ) are shown for each dotted line. K D = K dis / K a .

    Journal: Journal of Virology

    Article Title: Somatic Hypermutation and Framework Mutations of Variable Region Contribute to Anti-Zika Virus-Specific Monoclonal Antibody Binding and Function

    doi: 10.1128/jvi.00071-22

    Figure Lengend Snippet: Kinetic analysis of anti-ZIKV MAbs. Kinetic analysis was conducted by Octet HTX (Sartorius). Anti-ZIKV MAbs were conjugated to an amine-reactive 2nd generation (AR2G) biosensor at 0.1 to 0.3 μg/mL, and the association constant ( k a ) was measured over 0 to 900 s and dissociation constant ( k dis ) for 1,200 s for ZIKV-VLP or E proteins at various concentrations. (A) MAb 289-3 and 102-1 to ZIKV-VLP, 3.3 to 16.7 nM. (B) MAb 289-3 and 102-1 to ZIKV E proteins, 6.67 to 33.3 nM, red line, fitting pattern. (C) k a / k dis plot for anti-ZIKV MAbs to ZIKV-VLP and ZIKV E proteins. Blue, MAb 78-2, 289-3, 306-2 and 278-11; red, MAb 102-1, 242-3. 270-12, and 11-3. k a and k dis values were calculated by two runs, and the average values are shown. Values (nM) for equilibrium dissociation constants ( K D ) are shown for each dotted line. K D = K dis / K a .

    Article Snippet: DENV-1-VLP (Nauru/Western Pacific/1974), DENV-2-VLP (Thailand/16681/84), DENV-3-VLP (Sri Lanka D3/H/IMTSSA-SRI/2000/1266), DENV-4-VLP (Dominica/814669/1981), ZIKV-VLP (Suriname Z1106033), and ZIKV E protein (Suriname Z1106033) were purchased from The Native Antigen Company (Oxford, UK).

    Techniques:

    Binding activity of framework region (FWR) amino acid reverted anti-ZIKV MAb. (A to D) Association and dissociation analysis of FWR amino acid reverted anti-ZIKV MAbs by Octet HTX (Sartorius); 2 μg/mL MAbs were captured to protein G biosensor (Sartorius), and 3 μg/mL ZIKV E protein was associated for 600 s and dissociated for 900 s. (E to H) Reactivity of anti-ZIKV MAbs of ZIKV-VLP using Luminex assay. Blue, anti-ZIKV MAbs with matured amino acid; red, anti-ZIKV MAbs with amino acid reverted to allele. (A and E) MAb 102-1. (B and F) MAb 270-12. (C and G) MAb 289-3. (D and H) MAb 306-2.

    Journal: Journal of Virology

    Article Title: Somatic Hypermutation and Framework Mutations of Variable Region Contribute to Anti-Zika Virus-Specific Monoclonal Antibody Binding and Function

    doi: 10.1128/jvi.00071-22

    Figure Lengend Snippet: Binding activity of framework region (FWR) amino acid reverted anti-ZIKV MAb. (A to D) Association and dissociation analysis of FWR amino acid reverted anti-ZIKV MAbs by Octet HTX (Sartorius); 2 μg/mL MAbs were captured to protein G biosensor (Sartorius), and 3 μg/mL ZIKV E protein was associated for 600 s and dissociated for 900 s. (E to H) Reactivity of anti-ZIKV MAbs of ZIKV-VLP using Luminex assay. Blue, anti-ZIKV MAbs with matured amino acid; red, anti-ZIKV MAbs with amino acid reverted to allele. (A and E) MAb 102-1. (B and F) MAb 270-12. (C and G) MAb 289-3. (D and H) MAb 306-2.

    Article Snippet: DENV-1-VLP (Nauru/Western Pacific/1974), DENV-2-VLP (Thailand/16681/84), DENV-3-VLP (Sri Lanka D3/H/IMTSSA-SRI/2000/1266), DENV-4-VLP (Dominica/814669/1981), ZIKV-VLP (Suriname Z1106033), and ZIKV E protein (Suriname Z1106033) were purchased from The Native Antigen Company (Oxford, UK).

    Techniques: Binding Assay, Activity Assay, Luminex